BT
中文

Cell Line Engineering

Comprehensive gene regulation cell line construction services

Regulation Type Technical Route
Gene Knockout (KO) CRISPR/Cas9 dual nickase or RNP strategy
Endogenous Activation (CRISPRa) dCas9-VPR / dCas9-SAM system
Endogenous Inhibition (CRISPRi) dCas9-KRAB system
Site-Directed Mutagenesis ssODN/HDR template + CRISPR knock-in strategy
Site-Directed Methylation dCas9-DNMT3A fusion system
Site-Directed Demethylation dCas9-TET1 fusion system
Conventional Overexpression Lentiviral / transient transduction expression vector
Conventional Knockdown shRNA / miRNA expression vector

Technical route feasibility depends on gene locus and cell type. Specific protocols require technical consultation to confirm.