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Perturb-seq · CRISPR Screening · Cell Line Engineering

From gene editing to single-cell omics, full-process technical solutions for researchers

Perturb-seq Full-Process Platform

01

sgRNA Design

Algorithm-based design covering target gene regions using genomic databases

02

Lentiviral Library Construction

Standardized library packaging and titer determination workflow

03

Cell Model Transduction

CRISPR perturbation transduction for multiple cell types

04

Single-Cell Capture & Sequencing

Single-cell isolation and library construction on 10x Genomics platform

05

Data Analysis & Visualization

Complete bioinformatics pipeline from raw data to perturbation-phenotype association analysis

CRISPR single-cell screening, gene knockout screening, CRISPRa screening, CRISPRi screening, tumor immune target discovery, synthetic lethality screening

From Perturbation to Model: A Technical Pathway for High-Throughput Genotype-Phenotype Data

The convergence of high-throughput CRISPR screening and single-cell sequencing technologies enables systematic construction of "gene perturbation—transcriptomic response" association datasets at the whole-genome scale. Such datasets serve as an important foundation for developing cell-computation-based predictive models.

We are committed to providing researchers with high-quality, traceable Perturb-seq data production and analysis methods, supporting exploration in this field.